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Chromatography medium volume calculators
Gel filtration |
Affinity, Expanded bed adsorption, Hydrophobic interaction, Ion exchange, Plasmid purification and Reversed phase |
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* in case the resolution is insufficient please increase the amount of chromatography medium
The chromatography medium volume is calculated from the formula:
The chromatography medium volume (L) = Sample volume to be processed (L) / (Sample volume in percent of total column volume (%) / 100)
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* refer to general guidelines in the "Column and system for scale" section, see left-hand navigator
The chromatography medium volume is calculated from the formula:
The chromatography medium volume (L) =(Quantity to be bound (g) * (1+ safety margin (%) / 100)) / (Dynamic binding capacity (g/L)
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Calculate your packed bed volume, settled chromatography medium volume and slurry volume
Please check that you have sufficient chromatography medium for the required packed bed volume by calculating the appropriate settled chromatography medium volume. Also ensure you have necessary tank capacity for the medium slurry by calculating the slurry volume. When packing Chromaflow columns we recommend to prepare approximately 5% extra slurry to prevent getting air in the column.
Below given compression factors are approximations that can vary slightly with applications.
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| Media | Compression factor | Recommended slurry concentration |
| Affinity |  | |
| Blue Sepharose 6 Fast Flow | 1.15 | 50-70% |
| Chelating Sepharose Fast Flow | 1.15 | 50-70% |
| Heparin Sepharose 6 Fast Flow | 1.15 | 50-70% |
| IMAC Sepharose 6 Fast Flow | 1.15 | 50-70% |
| MabSelect | 1.15 | 50-70% |
| MabSelect SuRe | 1.15 | 50-70% |
| MabSelect Xtra | 1.15 | 50-70% |
| Ni Sepharose 6 Fast Flow | 1.15 | 50-70% |
| Expanded bed adsorption | | |
| STREAMLINE DEAE | 1.0 | ~70% |
| STREAMLINE HST | 1.0 | ~70% |
| STREAMLINE Q XL | 1.0 | ~70% |
| STREAMLINE rProtein A | 1.0 | ~70% |
| STREAMLINE SP | 1.0 | ~70% |
| STREAMLINE SP XL | 1.0 | ~70% |
| Gel filtration | | |
| Sephacryl S-100 HR | 1.15 | 50-70% |
| Sephacryl S-200 HR | 1.15 | 50-70% |
| Sephacryl S-300 HR | 1.15 | 50-70% |
| Sephacryl S-400 HR | 1.15 | 50-70% |
| Sephacryl S-500 HR | 1.15 | 50-70% |
| Sephadex G-25 Coarse | 1.2 | 50-70% |
| Sephadex G-25 Fine | 1.2 | 50-70% |
| Sephadex G-25 Medium | 1.2 | 50-70% |
| Sephadex G-25 Superfine | 1.2 | 50-70% |
| Sepharose 4 Fast Flow | 1.15 | 50-70% |
| Sepharose 6 Fast Flow | 1.15 | 50-70% |
| Superdex 30 prep grade | 1.15 | 50-70% |
| Superdex 75 prep grade | 1.15 | 50-70% |
| Superdex 200 prep grade | 1.15 | 50-70% |
| Hydrophobic interaction | | |
| Butyl Sepharose 4 Fast Flow | 1.15 | 50-70% |
| Butyl Sepharose High Performance | 1.15 | 30-50% |
| Butyl-S Sepharose 6 Fast Flow | 1.15 | 50-70% |
| Octyl Sepharose 4 Fast Flow | 1.15 | 50-70% |
| Phenyl Sepharose 6 Fast Flow (high sub) | 1.15 | 50-70% |
| Phenyl Sepharose 6 Fast Flow (low sub) | 1.15 | 50-70% |
| Phenyl Sepharose High Performance | 1.15 | 30-50% |
| SOURCE 15 ETH | 1.05 | 30-50% |
| SOURCE 15 ISO | 1.05 | 30-50% |
| SOURCE 15 PHE | 1.05 | 30-50% |
| Ion exchange | | |
| Capto DEAE | 1.1 | 50-60% |
| Capto MMC | 1.1 | 50-60% |
| Capto Q and Capto ViralQ | 1.1 | 50-60% |
| Capto S | 1.1 | 50-60% |
| CM Sepharose Fast Flow | 1.15 | 50-70% |
| DEAE Sepharose Fast Flow | 1.15 | 50-70% |
| MacroCap SP | 1.18 | 60-65% |
| Q Sepharose Big Beads | 1.15 | 50-70% |
| Q Sepharose Fast Flow | 1.15 | 50-70% |
| Q Sepharose High Performance | 1.15 | 30-50% |
| SOURCE 15 Q | 1.05 | 30-50% |
| SOURCE 15 S | 1.05 | 30-50% |
| SOURCE 30 Q | 1.05 | 30-50% |
| SOURCE 30 S | 1.05 | 30-50% |
| SP Sepharose Big Beads | 1.15 | 50-70% |
| SP Sepharose Fast Flow | 1.15 | 50-70% |
| SP Sepharose High Performance | 1.15 | 30-50% |
| Mab purification | | |
| Capto adhere | 1.1 | 50-60% |
| Capto Q | 1.1 | 50-60% |
| Capto S | 1.1 | 50-60% |
| MabSelect | 1.15 | 50-70% |
| MabSelect SuRe | 1.15 | 50-70% |
| MabSelect Xtra | 1.15 | 50-70% |
| Plasmid purification | | |
| PlasmidSelect | 1.15 | 50-70% |
| PlasmidSelect Xtra | 1.15 | 30-50% |
| Reversed phase | | |
| SOURCE 15 RPC | 1.05 | 30-50% |
| SOURCE 30 RPC | 1.05 | 30-50% |
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