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Packing procedures
You will find a suggested packing technique by giving the chromatography medium, bed height and column in use. The suggested technique is to be considered as a starting point. The most optimal packing technique may differ for your application. Before starting to pack columns with diameters of 400 mm and above we advice you to contact your local
GE Healthcare technical support team.

General advice to achieve a good packing
Before packing the column please make sure that
> the tubing, valves, gaskets etc. have the same inner diameter as the column in- and outlets and tubing is not longer than needed.
> the bed supports have a porosity appropriate for your chromatography medium
> the column has been correctly assembled verified by passing a leakage test
> the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment
> that the pump is free from pulsation
> that you have enough medium, taken into account the compression factor
> packing is performed at optimal flow velocity
> column meets the pressure requirements for selected medium and packing procedure


Select technique of interest:
Affinity
Gel filtration
Hydrophobic interaction
Ion exchange
Mab purification
Plasmid purification