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Protein & DNA Expression Workflow

Construction of expression-ready DNA clones
Overexpression & purification of specific protein(s), structure & function studies

Click on workflow diagram for corresponding Sample Preparation category pages.

Sample(s): Cells, bacteria, yeast, etc. Plasmid DNA Preparation
Protein Expression
Protein Capture and Cleavage of Tag
Mass Spectrometry. Characterized Biomolecule: e.g. Biomarker
Plasmid DNA Purification Rolling Circle Amplification Lysis/Tissue Homogenization Sequencing Enzyme Regulation Cloning GST-tagged Protein Capture 1-D Electrophoresis Histidine-tagged Protein Capture Desalting / Buffer Exchange / Clean-up MBP-tagged Protein Capture Concentration Liquid Chromatography Strep(II)-tagged Protein Capture Desalting / Buffer Exchange / Clean-up Concentration Protein Interactive Analysis