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A simple and quick method for successful sequencing of difficult genomic regions

The illustra™ TempliPhi™ Sequence Resolver Kit produces exceptional sequencing results from difficult templates such as those with high GC content and secondary structures. The kit resolves most of ...

Amplification of DNA from diverse sources with the illustra GenomiPhi HY DNA Amplification Kit

The illustra™ GenomiPhi™ HY DNA Amplification Kit simplifies the process of genomic DNA preparation and stock replenishment and makes it easier to prepare multiple genomic DNA samples simultaneously. ...

Amplification of Plant DNA with GenomiPhi DNA Amplification Kit

Polysaccharide and polyphenol compounds in plants can interfere with DNA extraction. GenomiPhi DNA Amplification Kit offers a way to prepare microgram quantities of DNA from leaves and seeds. Often ...

Amplified DNA for use in STR analysis

This application notes describes the use of GenomiPhi DNA Amplification Kit to amplify human genomic DNA and the genotyping of the amplified and purified DNA for 400 STR loci.

Application Note #1: Heteroduplex mobility assay for subtyping HIV-1

HIV-1 has been classified into 8 subtypes (A-H and O) on the basis of phylogenetic comparisons of the sequences of the env and gag genes. Subtypes circulating in particular geographical regions have ...

Application Note: DNA Fragment Analysis Using PhastGel Media

PhastSystem, in combination with thin, ready-made PhastGel media and the PhastGel DNA Silver Staining Kit, provides rapid, convenient and reproducible electrophoresis of DNA fragments Rapid, high ...

Application Note: SSCP analysis of PCR products to identify clones in lymphoid leukaemia and lymphoma

SSCP can be detected with a non-isotopic electrophoretic technique using PhastSystem™ and sensitive silver staining. The advantages of this method include: Rapid; a result 2 hours after completion of ...

Application Note: SSCP analysis of PCR products to type human papillomavirus

SSCP can be detected with a non-isotopic electrophoretic technique using PhastSystem ™ and sensitive silver staining. HPVs are recognised as important agents of disease. Increasingly sophisticated ...

Application Note: SSCP analysis of PCR products to type the coagulation factor V gene

Resistance to activated protein C (APC) is associated with venous thromboembolism. The APC-resistance is to 90% due to a point mutation in the factor V gene (G to A at nucleotide position 1691). The ...

Applications of PuReTaq Ready-To-Go PCR Beads in Real-Time PCR

This application note demonstrates the ability of PuReTaq Ready-To-Go PCR Beads to deliver low background ampliﬁcation while maintaining the same PCR efﬁciency, ﬂexibility, and reproducibility found ...

Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor

GE Healthcare offers a range of DNA amplification products to meet all genomic DNA and sequencing template preparation needs. A combination of these kits with the Genesis Freedom™ Robotic Sample ...

Characteristics of new short gel filtration columns

Gel filtration (GF) is a gentle technique suitable for the study of aggregation and purity of a target protein. GF, however, is often time consuming and sample and buffers can be expensive. A new, ...

CodeLink Bioarray image threshold flagging procedure when using the Agilent G2565BA scanner

Signal strength was found to be different on the Agilent G2565BA scanner from that on the Molecular Devices GenePix™ 4000B scanner, making it necessary to adjust the threshold accordingly. It was ...

Evaluation of the performance of Amersham HyPer5 dye in comparative genomic hybridization microarray applications

The labeling efficiency of HyPer™5 fluorescent dye was compared to that of Amersham Cy™5-dCTP and Amersham Cy3-dCTP in three different experiments. The labeled probes were then used in 3K bacterial ...

Fluorescence Quantitation of PCR Products Using the DyNA Quant 200 Prior to Re-amplification and Direct Sequencing

Determination of the concentration of PCR products is important for automated DNA sequencing, gel electrophoresis analysis and molecular cloning. Yet there are several limitations in determining the ...

Fluorescent DNA Labeling by PCR

Amplification of DNA using the polymerase chain reaction (PCR) provides the opportunity to label the resulting product with either modified nucleotides or oligonucleotide primers. Incorporation of ...

GenomiPhi amplification of FFPE tissue DNA for array CGH

Human genomic DNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue samples was amplified using the GenomiPhi™ DNA Amplification Kit, fluorescently labeled with Cy™3 and Cy5 dyes, and ...

Improved method of BAC DNA preparation for sequencing

Bacterial artificial chromosomes (BACs) are cloning vectors based on the F factor plasmid of Escherichia coli. They are capable of maintaining cloned DNA fragments up to 300 kb (1). Sequencing of BAC ...

Increased polymerase chain reaction (PCR) amplification specificity using illustra Hot Start Master Mix

Hot Start Master Mix blocks polymerase activity using primer sequestration to a level comparable to those seen for direct inhibition using anti-Taq monoclonal antibody or chemical modification. Hot ...

Isolation of total RNA with the illustra RNAspin 96 Isolation Kit

The illustra™ RNAspin 96 Isolation Kit can be used to reproducibly (well-to-well, plate-to-plate) isolate total RNA from cultured cells, tissue, bacteria or yeast. It allows for the simultaneous ...

Isolation of total RNA with the illustra RNAspin Midi Isolation Kit

The illustra™ RNAspin Midi Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria and yeast. The procedure is straightforward and takes less than 30 min to complete. The ...

Isolation of total RNA with the illustra RNAspin Mini Isolation Kit

illustra™ RNAspin Mini Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria or yeast. The isolated RNA is of a high enough quality and quantity (up to 100 �g) for ...

Evaluation of microarray hybridization data using Universal ScoreCard with oligonucleotide probes

Universal ScoreCard consists of a set of 23 artificial genes that serve as analytical controls to validate and normalize microarray data obtained from array hybridization. The genes have been ...

New column format for process development

HiScreen™ columns are part of the process development platform available from GE Healthcare. The columns are prepacked with a range of BioProcess™ media and designed for method optimization and ...

Oncogene mRNA Profiling Using Fluorescent Quantitative PCR

The regulated expression of oncogene sequences is essential for normal cell growth, differentiation, and function. Altered expression of these genes can lead to cell transformation. Several ...

Rapid protein analysis and screening of membrane protein buffer conditions by gel filtration

Screening of different conditions is often necessary to obtain protocols that result in pure and stable target proteins. Sizeexclusion chromatography (SEC) is an excellent and gentle technique to ...

Rapid screening of antibody aggregates and protein impurities using short gel filtration columns

Purification of antibodies often generates dimers and higher aggregated forms and during optimization of purification protocols, many samples may need to be analyzed for aggregate content. Gel ...

The SCIANTIS System - an interactive, online encyclopedia for gene expression analysis

The SCIANTIS™ System from Gene Logic is a fast and affordable online research tool to assess, prioritize, and qualify gene targets and biomarkers across major therapeutic areas. Comprising thousands ...

Transformation of plasmid DNA amplified with TempliPhi DNA Amplification Kits

TempliPhi DNA Amplification Kits use a novel isothermal process to efficiently prepare DNA sequencing templates in as little as 4 h. Circular DNA templates are amplified by rolling circle ...

Use of amplified DNA as a substrate for multiplex PCR

The amplified DNA from GenomiPhi Kit can substitute for genomic DNA in many downstream applications. Since many genomic DNA applications begin with PCR amplification of a specific fragment, we have ...

Use of amplified DNA as a substrate for uniplex PCR

This application note demonstrates that uniplex PCR from the same mass of ampliﬁed DNA and genomic DNA perform identically. In addition, there is no need to purify the GenomiPhi product prior to PCR.

Use of amplified DNA with GeneChip HuSNP Mapping Assay

SNP scoring is a major effort in laboratories performing genetic variation research such as disease association studies. These experiments are also a major consumer of genomic DNA due to the number ...

Use of GenomiPhi DNA Amplification Kit for array CGH

GenomiPhi™ DNA Amplification Kit was compared with DOP-PCR for the amplification of BAC DNA to fabricate microarray slides for use in comparative genomic hybridization (array CGH). The GenomiPhi ...

Whole genome amplification from blood cards

Blood cards are a convenient solution for collecting low volume blood samples such as those from neonates and for long term storage at ambient temperatures for archival purposes. The amount of ...

Whole genome amplification from crude blood lysates

Whole blood is a common source of DNA for genetic analysis, but isolating genomic DNA from blood often requires extensive purification steps. This application note describes a simple, robust method ...

Whole genome amplification, cloning, and expression of an exonuclease III gene

This application note describes DNA amplification and production of a shotgun library from Thermoplasma volcanium glycerol stock without culturing the organism. The successful cloning and expression ...

Whole genome amplification from cheek cells

Buccal swabs provide a low-cost, non-invasive way to collect genomic DNA. This is also a popular method because refrigeration is not required and the DNA remains usable after years of storage. ...

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