Nucleic acid blotting
Following electrophoresis, Southern or Northern blotting involves the transfer and immobilization (blotting) of nucleic acids from the gel to a solid support (membrane). Neutral or positively-charged nylon are the most common types of membrane used for nucleic acid transfer. Blotting allows the use of detection by probe hybridization.
The conventional method of blotting is capillarity transfer, although vacuum blotting is growing in popularity due to the fast and even nature of the transfer.
Capillary Blotting
The agarose gel is mounted onto a paper wick that dips into a reservoir containing transfer buffer. The membrane is sandwiched between the gel and a stack of absorbent material that serves to draw the transfer buffer through the gel by capillary action. The nucleic acid molecules are carried through the gel by the buffer and become immobilized on the membrane
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