Kinase adenoviral vector gene delivery system
Ad-A-Gene vectors allow GFP-kinase translocation assays to be easily established in a wide range of cell types. These convenient, easy to use reagents expand the possibilities for drug target validation and lead compound profiling.
Kinase Ad-A-Gene delivery system |
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Adenoviruses are a family of DNA viruses. They are used as a vehicle for the delivery of genes in gene-therapy and for protein expression in cultured cells.
Adenoviral vector entry into the cell, or transduction, involves a number of interactions between proteins on the capsid coat of the vector and target cell surface. The process starts with absorption of the virus onto target cells through interaction of the knobbed fibre protein to the high affinity coxsackie and adenovirus receptor (CAR) on the cell surface. Entry of the adenoviral vector is mediated by interaction of the capsid penton base with cell surface integrins. After these interactions, the vector is internalised through clathrin mediated endocytosis. The pH in the endosome causes release of the virus into the cytoplasm. Transport to the nucleus then proceeds where DNA replication and transcription occur from an epichromosomal location, resulting in expression of the gene of interest (GOI).
Ad-A-Gene adenoviral vectors provide an easy to use, cost-effective system for mammalian gene delivery to cultured cells due to their stability and ability to direct high level transient gene expression in a wide variety of cell types, including primary cells.
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