ADP accumulation assays
Fluorescent assays for kinase activityADP accumulation assays profile enzyme activity of kinases and ATPases by employing a fluorescent non-antibody based method to detect the amount of ADP. The ADP resulting from the kinase reaction is reused as a co-factor in a subsequent enzymatic reaction to catalyze the production of a readable fluor.
Kinase ADP accumulation fluorescent assays |
| Product name | | | ADP Hunter HS
Coming soon | |
| Application | Profiling of enzyme activity and inhibitor studies | Profiling of enzyme activity and inhibitor studies | High throughput screening of kinase inhibitors | High throughput screening of kinase inhibitors |
| Assay protocol available |
Kinetic
Endpoint |
Kinetic
Endpoint |
Endpoint |
Endpoint |
| Assay sensitivity | 0.3 µM ADP | 0.5 µM ADP | 0.3 µM ADP | 0.6 µM ADP |
| Linear range | 0-10 µM ADP | 0-25 µM ADP | 0-10 µM ADP | 0-25 µM ADP |
| Dynamic linear range | Up to 20 µM | Up to 75 µM | Up to 20 µM | Up to 75 µM |
| [ATP] in assay | Less than 50 µM | Up to 200 µM | Less than 50 µM | Up to 200 µM |
| Tolerance to DTT | 0.005 mM | 0.05 mM | 2 mM | 2 mM |
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ADP Quest HS and ADP Quest are useful tools to determine Michaelis-Menten kinetics and to profile inhibitor mode of action and potencies.
ADP Hunter HS and ADP Hunter PLUS minimize compound library interference and contains a stop solution to stabilize the reaction at a given endpoint for longer reads.
TK Subtrate Quest provides a simple method to screen up to 22 subtrates (8 replicates each) designed for phosphorylation by tyrosine kinases.
Select another in vitro kinase assay | 
