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in vitro kinase assays
HitHunter Enzyme Fragment Complementation (EFC) kinase activity and kinase binding assays are robust and reliable antibody-based chemiluminescent platforms specifically designed for high throughput screening. The HitHunter EFC assays provide a rapid and homogeneous methodology for detecting intracellular analytes in 384-well format microplates.
ADP accumulation assays are a fluorescent non-antibody-based platform that is compatible with any kinase or ATPase reactions. Withstand of ATP concentrations up to 200 µM and their 384-well format make the ADP accumulation assays a top choice for automated high throughput screening.
Scintillation proximity assays (SPA) is a format-independant platform that relies on microscopic beads emitting light proportionally to the proximity of radioactive stimulation. SPA achieves high-sensitivity out of standard scintillation counters with smaller amounts of radioactive meterials when compared to other radioactive methods.
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