Chemifluorescence

Chemifluorescence is an easy-to-use technique for nonradioactive DNA, RNA, and protein detection on blots. With chemifluorescence, there is no exposure step to wait for -- the Storm system reads your chemifluorescence blots in minutes. Quantitative analysis is simplified with chemifluorescence too, because there's no film intermediate.

Southern blotting with chemifluorescence Bands were visualized by chemifluorescence in this Southern blot of human genomic DNA.

How chemifluorescence works

Fig 2. Alkaline phosphatase cleaves a phosphate group from the chemifluorescent substrate, releasing a highly fluorescent product. The product absorbs 450 nm light, and emits light at 540-560 nm.

Easy, familiar protocols

Chemifluorescence sample preparation is very similar to chemiluminescent sample procedures, but chemifluorescence produces a stable, fluorescent reaction product -- so you can scan your blot at your own convenience. Chemifluorescence works with strip and re-probe procedures and doesn't require you to change your hybridization conditions.

ECL Plus western blots with no change in protocol

Although it is most commonly used in chemiluminescence procedures, Storm sensitively detects the fluorescence signal produced by the ECl Plus substrate. ECL Plus Western blotting protocols are based on HRP-conjugated antibodies. This offers a useful complement to the AP-conjugates used in chemifluorescence.

Storm reads chemifluorescence right off the blot

• no guessing when to expose- chemifluorescence is stable for weeks
• no over or under-exposures
• no film intermediate to complicate quantitation
• no darkroom processing