The only chemiluminescent and chemifluorescent solution for Western blotting
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ECL Plus™ Western Blotting Detection Systems deliver superior sensitivity with the added advantage of chemifluorescent detection on an appropriate scanner.
This versatile functionality enables you to carry out qualitative analysis (on film) and semiquantitative analysis using CCD imagers or laser scanners with single antibody or stripping and reprobing.
The acridan-based substrate used in ECL Plus Western Blotting Detection Systems releases a high level sustained output of light and, in addition, generates an intense chemifluorescent signal at 440 nm.
This chemifluorescent signal can be detected on a fluorescence imager with the appropriate filters.
- Higher sensitivity (~5 pg in model system using film, ~40 pg using imager)
- Chemiluminescent and chemifluorescent detection
- Extended signal duration (up to 48 hours) enables multiple exposures to be made
- Compatibility with Hybond™ ECL™ and Hybond-P membranes (Hybond-P is more robust for stripping and reprobing)
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