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ECL Western Blotting Systems
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TECHNICAL SPECIFICATIONS - ECL Western Blotting Systems
	ECL Plex™	ECL Advance™	ECL Plus™	ECL™
Sensitivity (pg)	~ 1	~ 1	~ 5	~ 10
Primary antibody usage	1 : 750	1 : 100 000	1 : 10 000	1 : 1 500
Secondary antibody dilution	1 : 2500	1 : 400 000	1 : 100 000	1 : 10 000
Emission duration (h)	> 3 months, protected from light	4-6	24-48	1-2
Reprobing possible	Not necessary	Yes	Yes	Yes
Recommended membrane for blotting	Hybond™-LFP Hybond ECL	Hybond-P Hybond ECL	Hybond-P	Hybond ECL
Recommended detection method	Fluorescence scanner	Hyperfilm™ ECL CCD imaging	Fluorescence Scanner Hyperfilm ECL CCD imaging	Hyperfilm ECL, CCD