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Quantitative results with ECL Plex
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Targets: phosphorylated p38 (pp38) and human actin in T293 cells stimulated 0, 2.5, 5, or 15 min with TGF-b, phosphorylated and total protein detected.
Detection: Hybond™-LFP on Typhoon™ 9410.
Primary antibodies: polyclonal anti-phospho-p38 MAP kinase (Thr 180/Tyr 182) and monoclonal anti-actin.
Secondary antibodies: ECL Plex™ goat-a-rabbit IgG-Cy5 + ECL Plex goat-a-mouse IgG-Cy3. The relative intensity is the scored intensity value of pp38 when quantitated relative to the total amount of anti-actin.
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