Introduction to enzyme fragment complementation (EFC)
HitHunter Assays are based on enzyme fragment complementation (EFC). This technology utilizes a genetically engineered beta-galactosidase (ß-gal) enzyme consisting of two fragments— a large protein fragment that serves as an enzyme acceptor (EA) and a small peptide fragment that serves as an enzyme donor (ED).
When the two fragments are separated, the enzyme is inactive. But together in solution, the fragments recombine spontaneously to form an active enzyme by a process called "fragment complementation". The recombined beta-galactosidase hydrolyzes luminescent or fluorescent substrates to produce an easily detectable signal.
Product selection guide — Select the best high throughput screening assay for your needs | 
