Proteases — HitHunter EFC assays
Enzyme Fragment Complementation for protease activity
Protease EFC Chemiluminescence HitHunter Assays |
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In the HitHunter™ Protease Assays, enzymes that recognize the Protease cleavage sequence will cleave the enzyme donor (ED) cyclic conjugate into a linear form, allowing it to recombine with the enzyme acceptor (EA) to form an active ß-galactosidase enzyme. The now active ß-galactosidase hydrolyzes the luminescent substrate to produce an easily detectable chemiluminescent signal
Proteases participate in a wide variety of physiological processes by controlling complex proteolytic cascades. Many disease states which manifest as altered protease expression and substrate proteolysis are targets for therapeutic intervention. Protease inhibitor drugs, like ACE inhibitors and HIV inhibitors, act by inhibiting key proteases. Current efforts to design and screen for therapeutic protease inhibitors are being accerlerated by the availability of structural information and several assay formats to screen, especially in the area of anticoagulation, neurodegenerative conditions, osteoporosis, type II diabetes and rheumatoid arthritis.
Product selection guide — Select the best high throughput screening assay for your needs
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