Kinases — HitHunter EFC assays
Enzyme Fragment Complementation for kinase activity
HitHunter Kinase Assays use standard kinase reaction conditions followed by two simple reagent additions for EFC detection.
Kinase EFC Chemiluminescence HitHunter Assays |
| | |
|
HitHunter EFC Assays for functional kinase activity are an antibody-based competitive binding format that uses EFC chemiluminescent detection. HitHunter EFC detection provides sensitivity through signal amplification. The EFC signal generated is proportional to the amount of phosphopeptide generated by the kinase enzyme.
Each fully configured kit includes:
- Phosphorylated kinase substrate-specific antibody
- Phosphorylated kinase substrate
- Phosphorylated kinase substrate control
- Non-phosphorylated kinase substrate
- Enzyme acceptor (EA)
- Enzyme donor (ED)
- Chemiluminescent reagent (Cl)
- Assay buffer
Kinase enzyme, reaction cofactors, and microplates are not supplied.
A kinase reaction is composed of kinase substrate, enzyme, ATP, compound and any co-factor required for activating phosphorylation. Because the components of the kinase reaction are specific to each kinase, each component and incubation time for the kinase should be optimized. HitHunter EFC product inserts list the limits of common additives, solvents and concentrations that do not affect assay performance.
Product selection guide — Select the best high throughput screening assay for your needs
| 
