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Ficoll-Paque PREMIUM products
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Ficoll PM400
Percoll PLUS
Percoll
Density Marker Beads
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Cell separation with density gradient media

Ficoll-Paque™ PREMIUM products

Ficoll-Paque™ PREMIUM products
Reagents used to prepare different density preparations of mononuclear cells from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.

Manufactured according to GMP and ISO 13485:2003 standards and the recommendations of the United States Pharmacopeia.

Sterile and ready-to-use.

Ficoll-Paque PLUS

Ficoll-Paque PLUS
Ready to use sterile medium for isolation of human lymphocytes in high yield from peripheral blood.

Maintains viability and representative distribution of B and T lymphocytes.

Low levels of endotoxin (< 0.12 EU/ml).

Ficoll™ PM400

Ficoll™ PM400
For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.

Neutral, highly-branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.

Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/ml can be achieved.

Percoll™ PLUS

Percoll™ PLUS
For preparation of cells, subcellular particles and larger viruses. Percoll PLUS has verified low endotoxin level, and is non-toxic, sterile and re-sterilizable, even after adjustment to physiological ionic strength.

Gradients can either be preformed or spontaneously generated by centrifugation at moderate speeds in an angle-head rotor.

Iso-osmotic gradients throughout cover a range of densities up to 1.3 g/ml.

Percoll

Percoll
For separation of cells, subcellular particles and larger viruses (down to ~ 70S) under gentle conditions which preserve viability and morphological integrity.

Non-toxic to cells.

Adjustable to physiological ionic strength and pH.

Density marker beads
Density Marker Beads are coloured derivatives of Sephadex™ media each type being a modification of the basic cross-linked dextran matrix to produce beads having a specific, reproducible buoyant density in gradients of Percoll™/RediGrad™.

The beads are supplied freeze-dried, and unopened may be stored for up to 5 years at room temperature without change in properties.