Education - density gradient separation

Density Gradient Separation is a convenient and reliable technique for isolating and purifying cells, viruses and subcellular particles. By centrifuging the cell solution in a medium containing particles that form a gradient according to their sedimentation rate, cells can be separated according to their density (isopycnic centrifugation) or size (rate zonal centrifugation). The gradients can either be preformed or formed in situ.

In comparison with other techniques commonly used for cell separation (e.g. fluorescent cell sorters and magnetic beads), Density Gradient Separation offers some important advantages:

• No antibodies or reagents are needed in order to bind the cells to a matrix. Thus no such substances risk being brought along with the cells.
• No labeling of the cells is required. Your cells remain in their native state, the technique does not affect their receptors or genetic make-up.
• The method is fast and allows you to work with large volume