|
|||||||
|
|
|||||||
|
A toolbox of GE Healthcare chromatography media for MAb purification platforms
MabSelect SuRe™ enables best the platform approach due to its alkaline stability and enhanced protease resistance, which results in lower leakage levels of the ligand and generic elution profile. For the intermediate/polishing step, there are several suitable chromatography media based on different chromatographic techniques. One alternative is a strong multimodal anion exchanger represented by Capto™ adhere that makes it possible to design a two-step process, or followed by an anion exchanger, Capto Q, a three-step process. An alternative intermediate step is represented by cation exchange chromatography using Capto S for high capacity or SP Sepharose™ Fast Flow for high selectivity.. Read more about Optimization of loading conditions by using design of experiments (DoE). |