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BioProcess™ Glossary

Capture

The feed from the bioreactor is the crudest sample in the whole process and the capture stage will have great impact on all the following steps.

It is important to select the proper media very carefully. Resolution is normally achieved in step gradient mode using large-bead media. Selective binding, washing and step elution are important properties to study. Consider also cleaning.

In most cases, fast and efficient isolation of the target molecule from the feed is the main task. To handle the feed conveniently in a work schedule, total throughput must match feed volume and load. Identify chromatography media with high selectivity and high capacity that tolerate adequate flow rates. The following table helps you to choose the right technique and media for your capture step.



Capture Problem 
Technique
Large volume, diluted sampleGood,
If necessary reduce sample volume by ultrafiltration in advance 		Best,
if ligand available 		Avoid
High viscosityYes, 
using large beads or EBA 		Yes,
using large beads or EBA 		Yes,
using large beads or EBA
High ionic strengthYes, 
but reduce ion strength by dilution or use diafiltration in advance 		Yes,
may be reduce ion strength by dilution or use diafiltration in advance 		Best
High DNA/endotoxin contentYes,
use cation exchanger media 		Yes,
but reduce DNA/endotoxins in a pre-treatment, if CIP is a problem 		Good
Particulate manner/cell debris, cellsYes,
use EBA or microfiltration pre-treatment 		Yes,
use EBA or microfiltration pre-treatment 		Yes,
use EBA or microfiltration pre-treatment
Lipid contentYes,
use cation exchanger 		Avoid Avoid
ProteinasesDesign issue,
choose conditions with maximum selectivitly during binding 		Design issue,
choose conditions with maximum selectivitly during binding 		Design issue,
choose conditions with maximum selectivity during binding
Frequent NaOH required (CIP)Compatible Avoid or reduce CIP need Compatible