Quantitative Imaging Webinar

On June 18, 2008, GE Healthcare aired the webinar “Quantitative Imaging Of Western Blots And 2-D Electrophoresis Gels: Why Is It Important And How Do You Achieve It? Below is a link to the on-demand webinar to view at your convenience.

Webinar: Quantitative Imaging Of Western Blots And 2-D Electrophoresis Gels: Why Is It Important And How Do You Achieve It?

The archived version includes the 40 minute presentation and the following Q&A session.

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Detection of low abundant phosphorylated protein

Multiplexed detection of two proteins on the same blot enables reliable quantification relative to a house-keeping protein, eliminating experimental variation such as uneven sample loading. This image shows p38 activation upon TGF- stimulation for 0, 2,5, 5 or 15 minutes in 293T cells. Phosphorylated p38 was detected using ECL Plex Cy5 and the house-keeping protein actin was detected using ECL Plex Cy3.