illustra PuReTaq Ready-To-Go™ PCR Beads- Newly designed, premixed, predispensed reactions for PCR featuring high-performance PuReTaq DNA polymerase.
- Preformulated, predispensed, single-dose, ambient-temperature-stable beads ensure greater reproducibility between reactions, minimize pipetting steps and reduce the potential for pipetting errors and contamination.
- Use of PuReTaq DNA polymerase and other high-purity reagents ensures that each bead is free of contaminating DNA.
- Optimized for standard PCR, each bead yields a reaction containing ~ 2.5 units of PuReTaq DNA polymerase, 10 mM Tris-HCl, (pH 9.0 at room temperature), 50 mM KCl, 1.5 mM MgCl2, 200 μM of each dNTP, stabilizers, and BSA.
- Validated for real-time PCR with MGB Eclipse™ Probe Systems.
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illustra PuReTaq Ready-To-Go™ PCR Beads
Technical Information
illustra™ PuReTaq Ready-To-Go™ PCR Beads* are designed for performing standard PCR. The only additional reagents are water, template DNA and primers. The beads are provided in either 0.5 ml or 0.2 ml tubes that are compatible with most thermal cyclers. The 0.2 ml tubes come assembled in a 96-well (8 × 12) plate format that allows individual strips of eight tubes to be easily removed. This flexibility allows use of either the entire 96-well plate, strips of eight, or individual 0.2 ml tubes.
illustra PuReTaq Ready-To-Go™ PCR Beads use highly purified PuReTaq cloned Taq DNA polymerase. PCR amplifications were performed with two different commercial sources of “low DNA” Taq DNA polymerases; with native and cloned Taq DNA polymerases; and with PuReTaq DNA polymerase. Random primers were used without addition of template DNA. All reactions were amplified via standard PCR with 25 μl reactions: 95°C for 5 min followed by 40 cycles of 95°C for 30 s, 55°C for 30 s, and 72°C for 1 min. Reactions were analyzed by gel electrophoresis with 10 μl loading volumes.
Reproducibility of illustra PuReTaq Ready-To-Go™ PCR Beads versus original Ready-To-Go™ PCR Beads in real-time testing. The threshold value represents the PCR cycle in which fluorescence increases above background value.
* For licensing information, see Legal Info.
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