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2-D Electrophoresis
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Sample Preparation
1st Dimension - IEF
2nd Dimension - SDS
Instruments
Instrument Accessories
Reagents
Bind Silane
ReadySol IEF
AMPS
TEMED
SDS
Tris
Agarose NA
Molecular Weight Markers
Gel Staining
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Reagents

ReagentDescription
Use this first if your gel is to be attached onto a glass plate for subsequent spot picking.
Polyacrylamide Gel Ingredients
ReadySol, a solution of acrylamide and bisacrylamide, is convenient and fast with minimal preparation and handling.
Ammonium persulfate is the initiator of choice for the polymerization of acrylamide
TEMED is a catalyst for the polymerization of acrylamide when used with ammonium persulfate.
Sodium dodecyl sulfate, Sodium lauryl sulfate- an anionic detergent
Tris-(hydroxymethyl)-aminomethane – buffer for pH control
Sample application piecesUse to apply your molecular weight marker.
Use to seal your strips in place.



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