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Featured Products
GFP-PLCd-PH domain assay
This assay provides a live-cell screening resource for inducible regulation of PLCd- and PI(4,5)P2-mediated processes.
Cleavage of phosphoinositol lipids in the cell membrane constitutes a key event in signal transduction. The PH domain of PLCd associates with specific phosphoinositides at the inner surface of the plasma membrane in resting cells, and translocates transiently into the cytoplasmic space upon pathway stimulation.
The assay consists of an extensively validated stably transfected cell line and expression vector for use with automated and traditional confocal and epifluorescent microscopes.
Learn more about this assay
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Lipid Signaling
Mammalian cell membranes consist of relatively equal amounts of protein (often glycoprotein) and lipid. Lipids such as cholesterol affect the fluidity of biological membranes. One component, phosphatidylinositol, is pivotally involved in detecting, amplifying and transducing chemical signals on the surface of cells. Phospholipases are lipid modifying enzymes which utilize phospholipids to generate arachidonic acid. Arachidonic acid is a precursor of the eicosanoids (prostaglandins and leukotrienes) involved in inflammation. Phospholipase C (PLC) enzymes convert phosphatidylinositol biphosphate [P(4,5)IP2] into inositol triphosphate (IP3) and diacylglycerol, critical second messengers in Ca2+ mobilization and activation of Protein Kinase C. Phospholipase A2 (PLA2) isozymes hydrolyze phosphatidylcholine to produce lysophosphatidylcholine, a second messenger involved in cell proliferation and adhesion. Families of lipid kinases, such as the phosphatidylinositol 3-kinases (PI3 Kinase) and phosphatidylinositol 4-kinases (PI4 Kinase) also contribute to cellular signalling, cytoskeletal organization and vesicle budding through the activation PIP2 and phosphatidylinositol respectively.
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