 |
Cell signaling & gene reporting in vitro assays and reagents
Fluorescence and chemiluminescence Assays - (HitHunter™ Enzyme Fragment Complementation (EFC) Assays)
EFC technology provides a sensitive and homogeneous method for measuring analytes by enzymatically amplifying the signal. E.coli b-galactosidase (b-gal) has been split into two fragments: a large protein fragment, which acts as the enzyme acceptor (EA), and a small peptide fragment (~ 4-11 kD), which acts as the enzyme donor (ED). These fragments are inactive separately but, in solution, they rapidly recombine to form an active enzyme by the process of complementation. The recombined b-gal hydrolyses luminescent or fluorescent substrates to produce an easily detectable signal.
Choose one of the assays shown above to discover how the product can help in your studies into the cell signalling and gene reporting.
More information about HitHunter Enzyme Fragment Complementation (EFC)
Scintillation proximity assay (SPA)
Is an innovative approach for assay development and biochemical screening that allows the rapid and sensitive measurement of a wide variety of molecular interactions in a homogeneous system.
Microscopic beads contain a scintillant that can be stimulated to emit light. This stimulation event only occurs when radiolabeled molecules of interest are bound to the surface of the bead then blue light is emitted that can be detected on standard scintillation counters.
Choose one of the assays shown above to discover how the product can help in your studies into the cell signalling and gene reporting.
Need more information? Visit the SPA Website for a comprehensive overview |
|
