Recommended equipment for the kinases product range
Recommeded equipment and settings for the in vitro product range
Radiometric, fluorescent and chemiluminescent assays Can all be read on compatible CCD imagers.
The LEADseeker Multimodality Imaging System CCD imager can measure SPA Imaging assays, Cy Dye fluors, HitHunter™ chemiluminescent and fluorescent end point Enzyme Fragment Complementation Assays as well as a wide range of fluorescent and luminescent tags in 96, 384 and 1536 well microplates. Appropriate filter sets are available for all these end points.
Instruments settings for SPA Scintillation beads - MicroBetaTM |
 | PVT | YSi |
isotope | window setting | efficiency (%) | window setting | efficiency (%) |
3H | 5 - 320 | 55 | 5 - 500 | 80 |
33P | 5 - 600 | 60 | 5 - 750 | 37 |
35S | 5 - 600 | 57 | 5 - 700 | 52 |
125I | 5 - 530 | 49 | 5 - 650 | 87 |
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Instruments settings for SPA Scintillation beads - TopCountTM |
| PVT | YSi |
isotope | window setting | efficiency (%) | window setting | efficiency (%) |
3H | 1.5 - 35 | 34 | 0 - 50 | 69 |
33P | 1.6 - 150 | 47 | 2.9 - 256 | 30 |
35S | 1.5 - 150 | 40 | 2.9 - 256 | 43 |
125I | 1.5 - 100 | 33 | 0 - 100 | 75 |
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Instrumentation Settings |
TopCount | Version 4 software and above | scintillator – liquid/plastic
energy range – low
mode – normal | scintillator - glass energy range - low mode - high sensitivity |
TopCount | Version 3 software and below | scintillator – liquid
energy range – low
mode – high | scintillator - solid energy range - low mode - normal |
Other instruments e.g. RackBeta or Tri-Carb Liquid Scintillation Analyzer | Applicable software | wide open | wide open |
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Recommeded equipment and settings for the in vivo product range
GFP assays are widely used to study kinases in single-parameter assays performed on a variety of instruments, including standard fluorescence and confocal microscopes. GFP can also be used in higher throughput, multi-parameter assays performed on all high-content analysis systems including GE Healthcare's IN Cell Analyzer instruments.
New to Kinases? Get started with the basics
Not sure where to start? Consult the in vitro product selection guide to select the best product for your kinases in vitro application studies.
Not sure where to start? Consult the in vivo product selection guide to select the best product for your kinases in vitro application studies.
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