Product selection guide kinase in vitro assays
Please use the list below to determine the best product for your needs:
Unsure about which type of assay to use? Find out more about the techniques
Radiometric - Scintillation Proximity Assay (SPA)>
Radiometric - Filter capture>
Fluorescence and Chemiluminescence>
Scintillation proximity assay (SPA):
SPA is an innovative approach for assay development and biochemical screening that allows the rapid and sensitive measurement of a wide variety of molecular interactions in a homogeneous system.
Microscopic beads contain a scintillant that can be stimulated to emit light.This stimulation event only occurs when radiolabeled molecules of interest are bound to the surface of the bead then blue light is emitted that can be detected on standard scintillation counters.
Need more information? Visit the SPA website or a comprehensive overview
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Filter capture assay:
The Biotrak™ protein kinase C (PKC) provides a simple and reliable means of detecting PKC in a variety of samples. The system is based upon the PKC catalysed transfer of the - g-phosphate group of adenosine-5'-triphosphate to a peptide which is specific for PKC. The method has been optimised to exhibit maximum PKC activity.
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Fluorescence and Chemiluminescence- HitHunter™ Enzyme Fragment Complementation (EFC) Assays:
EFC technology provides a sensitive and homogeneous method for measuring analytes by enzymatically amplifying the signal. E.coli b-galactosidase (b-gal) has been split into two fragments: a large protein fragment, which acts as the enzyme acceptor (EA), and a small peptide fragment (~ 4-11 kD), which acts as the enzyme donor (ED).
These fragments are inactive separately but, in solution, they rapidly recombine to form an active enzyme by the process of complementation. The recombined b-gal hydrolyses luminescent or fluorescent substrates to produce an easily detectable signal.
Choose one of the assays shown above to discover how the product can help in your studies into kinases
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New to Kinases? Get started with the basics.
Not sure where to start? Consult the in vitro product selection guide to select the best product for your kinases in vitro application studies.
Not sure where to start? Consult the in vivo product selection guide to select the best product for your kinases in vitro application studies.
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