Product selection guide - in vitro assays, reagents and radioisotopes
Please click on the links below to find the appropriate product for your studies:
Choose one of the assays above to discover how the product can help in your studies into kinases
GE Healthcare supplies a wide range of kits and reagents to help you in your studies into kinases, we appreciate that you may not want to use a full kit or just a radioisotope, listed below are some options to help you decide which one to use:
More about:
SPA (Assay Kits and Reagents)>
Chemiluminescence kits>
Fluorescent Screening reagents (CyDyeTM)>
Biotinylated Myelin Basic Protein (bMBP)>
Radioisotopes>
SPA (Assay Kits and Reagent):
Scintilliation Proximity Assay (SPA). The principle of this assay (and other SPA beads) occurs when microscopic beads contain a scintillant can be stimulated to emit light. This event only occurs when radiolabeled molecules of interest are bound to the surface of the bead.
The p34cdc2 Protein Kinases SPA kit involves the transfer of [33P]phosphate from [33P]ATP to the tyrosine, serine, or threonine in the peptide or protein substrate. The phosphorylated peptide is then captured by a streptavidin-coated SPA bead.
Kit contents include: buffer, (including biotin-tagged peptide), streptavidin PVT SPA beads, stop solution and instructions for use.
Choose from the SPA assay or the entire range of SPA Beads
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Chemiluminescence kits:
The Chemiluminescence are part of the HitHunterTM range of Enzyme fragmentation complementation (EFC)assays
EFC technology provides a sensitive and homogeneous method for measuring analytes by enzymatically amplifying the signal.
Kit contents generally include: Assay and dilution buffer, standards antibody and reagents for luminescence detection methods.
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Fluorescent Screening reagents (CyDyeTM):
CyDye Fluors - offering bright and intense colours with narrow and spectrally distinct emission bands. CyDye Fluors are outstanding for multicolour detection in a range of applications.
Choose from a wide range of emission bands, mono-reactive malemide and ester and TRF reagents
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Biotinylated Myelin Basic Protein (bMBP):
Is an ideal substrate for studying the activity of kinases because it can be phosphorylated at multiple sites by a number of kinase enzymes (e.g. Protein kinase C [PKC], protein kinase A [PKA], and casein kinases II)
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Radioisotopes:
The basic principle of radiometric kinase assays involves the transfer of radiolabelled phosphate from [33P]- or [32P]- gATP to tyrosine, serine, or threonine residues in peptide or protein substrates. These products are ideal to use in your own experiments.
Choose from a 33P or 32P and colored stabilized solution (RedivueTM)
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New to Kinases? Get started with the basics.
Not sure where to start? Consult the in vitro product selection guide to select the best product for your kinases in vitro application studies.
Not sure where to start? Consult the in vivo product selection guide to select the best product for your kinases in vitro application studies.
View the support section for your technical support and customer service needs.
Understand how your instrumentation performs with our products.
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