Recommended Equipment for the GPCR Product Range
Radiometric, fluorescent and chemiluminescent assays Can all be read on compatible CCD imagers.
The LEADseeker Multimodality Imaging System CCD imager can measure SPA Imaging assays, Cy Dye fluors, HitHunter™ chemiluminescent and fluorescent end point Enzyme Fragment Complementation Assays as well as a wide range of fluorescent and luminescent tags in 96, 384 and 1536 well microplates. Appropriate filter sets are available for all these end points.
Instruments settings for SPA Scintillation beads - MicroBetaTM |
 | PVT | YSi |
isotope | window setting | efficiency (%) | window setting | efficiency (%) |
3H | 5 - 320 | 55 | 5 - 500 | 80 |
33P | 5 - 600 | 60 | 5 - 750 | 37 |
35S | 5 - 600 | 57 | 5 - 700 | 52 |
125I | 5 - 530 | 49 | 5 - 650 | 87 |
|
Instruments settings for SPA Scintillation beads - TopCountTM |
| PVT | YSi |
isotope | window setting | efficiency (%) | window setting | efficiency (%) |
3H | 1.5 - 35 | 34 | 0 - 50 | 69 |
33P | 1.6 - 150 | 47 | 2.9 - 256 | 30 |
35S | 1.5 - 150 | 40 | 2.9 - 256 | 43 |
125I | 1.5 - 100 | 33 | 0 - 100 | 75 |
|
Instrumentation Settings |
TopCount | Version 4 software and above | scintillator – liquid/plastic
energy range – low
mode – normal | scintillator - glass energy range - low mode - high sensitivity |
TopCount | Version 3 software and below | scintillator – liquid
energy range – low
mode – high | scintillator - solid energy range - low mode - normal |
Other instruments e.g. RackBeta or Tri-Carb Liquid Scintillation Analyzer | Applicable software | wide open | wide open |
|
CypHer 5E is widely used to study receptor internalization in single-parameter assays performed on a variety of instruments, including standard fluorescence and confocal microscopes. CypHer 5E can also be used in higher throughput, multi-parameter assays performed on all high-content analysis systems including GE Healthcare’s IN Cell Analyzer instruments.
New to GPCR? Get started with the basics.
Consult the product selection guide to select the best product for your GPCR application studies.
Consult the support section for your technical support and customer service needs
See how we can create custom solutions for your specific needs. | 
