HitHunter Enzyme Fragment Complementation Assays
Principle of the technology
HitHunter™ Enzyme Fragment Complementation (EFC) Assays are competitive binding assays for the sensitive and simple measurement of a range of biologically important targets. Based on the recombination of two inactive enzyme fragments, enzyme donor (ED) and enzyme acceptor (EA), to form the active tetrameric ß-galactosidase, the hydrolysis of fluorogenic or luminescent substrates generates a signal that can be easily read on the
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