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[
3
H] Serotonin Transporter Protein Assay
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Radiometric Application Sets
[
33
P] Lck Kinase Enzyme Assay
[
33
P] MAP Kinase Enzyme Assay
MMP-3 [
3
H] Enzyme Assay
NF-
k
B/p50 [
3
H] Enzyme Assay
[
3
H] Phosphodiesterase Enzyme Assay
[
3
H] Reverse Transcriptase Enzyme Assay
[
3
H] Serotonin Transporter Protein Assay
[
125
I] ß-amyloid 1-40 Aggregation Assay
[
125
I] MIP-1
a
Receptor Binding Assay
Fluorescence Application Sets
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[
3
H] Serotonin transporter protein assay
More about:
Assay concept>
1536-well assay protocol>
Reagent usage in 96-, 384- and 1536-well formats>
Typical data>
Ordering information>
Assay concept
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1536-well assay protocol
1
µ
l imipramine 10 mM
1
µ
l assay buffer (50 mM Tris pH 7.4, 120 mM NaCl, 5 mM KCl,)
3
µ
l [
3
H]imipramine (TRK553) 50 000 cpm
3
µ
l WGA yttrium oxide (
RPNQ0270
) beads (0.3
µ
g) precoupled to 10
µ
g receptor
Incubate overnight at 4
o
C
Image for 10 minutes
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Reagent usage in 96-, 384- and 1536-well format
Assay components
96-well
384-well
1536-well
Assay volume
200
µ
l
50
µ
l
8
µ
l
Bead
0.6
µ
g
0.6
µ
g
0.3
µ
g
Membrane amount
20
µ
g
20
µ
g
10
µ
g
Cold imipramine amount
200 nMoles
50 nMoles
10 nMoles
[
3
H] imipramine
100 000 cpm
100 000 cpm
50 000 cpm
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Typical data
aa
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Ordering information
The following products were used in this assay and can be purchased online
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Learn more about the
LEADseeker Imaging System,
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