[125I] MIP-1a receptor binding assay
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1536-well assay protocol>
Reagent usage in 96-, 384- and 1536-well formats>
Typical data>
Ordering information>
Assay concept
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1536-well assay protocol
1. 2 µl MIP-1a at 1.33 mM in assay buffer (50 mM HEPES, pH7.2, 1 mM MgCl2, 1 mM CaCl2)
2. 2 µl CCR1 receptor (3.13 µg)
3. 2 µl [125I] MIP-1a (IM285; 7.5 nCi/well)
4. 3 µl WGA polystyrene (RPNQ0260) beads (125 µg/well)
5. Seal the plate and incubate at room temperature for 120 minutes
6. Image for 10 minutes
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Reagent usage in 96-, 384- and 1536-well format
Assay components | 96-well | 384-well | 1536-well |
Assay volume | 200 µl | 40 µl | 9 µl |
Bead | 1 µg | 500 µg | 125µg |
Membrane amount | 25 µg | 12.5 µg | 3.125 µg |
Cold MIP-1a | 10-75 ng | 10-75 ng | 10-75 ng |
[125I] MIP-1a | 66 nCi | 33 nCi | 7.5 nCi |
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Typical data
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Ordering information
The following products were used in this assay and can be purchased online
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