[125I] ß-amyloid 1-40 aggregation assay
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384-well assay protocol>
Reagent usage in 96- and 384-well formats>
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Assay concept
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384-well assay protocol
- 48 µl containing: biotinylated ß-amyloid 1-40 peptide 0.25 µg, unlabelled ß-amyloid 1-40 peptide 1.25 µg, [125I] ß-amyloid (IM294) 1-40 peptide 15x103cpm, Pre-incubated at 370C for 5 mins prior to addition of: ZnCl2 100 mM, 50 mM Hepes pH7.5, 0.1% (w/v) BSA
- Streptavidin polystyrene beads (RPNQ0261) 12 µl (0.5 µg)
- Incubate for 3 hours at 37oC
- Incubate overnight at room temperature
- Image for 5 minutes
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Reagent usage in 96- and 384-well formats
Assay components | 96-well | 384-well |
Assay volume | 100 µl | 60 µl |
Bead | 1 µg | 0.5 µg |
ß1-40 amount | 2.5 µg | 1.25 µg |
Biotinylated ß1-40 amount | 0.5 µg | 0.25 µg |
[125I] ß1-40 | 0.023 µCi | 0.034 µCi |
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Typical Data
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Ordering information
The following products were used in this assay and can be purchased online
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