Product selection guide: Nuclear Receptor in vitro assays and reagents

Unsure about which type of assay to use? Find out more about the techniques:
Scintillation proximity assay (SPA)>
Fluorescence and Chemiluminescence>


Scintillation proximity assay (SPA)
SPA is an innovative approach for assay development and biochemical screening that allows the rapid and sensitive measurement of a wide variety of molecular interactions in a homogeneous system.

Microscopic beads contain a scintillant that can be stimulated to emit light.This stimulation event only occurs when radiolabeled molecules of interest are bound to the surface of the bead then blue light is emitted that can be detected on standard scintillation counters.

Choose one of the assays shown above to discover how the product can help in your studies into nucleor receptors

Need more information? Visit the SPA Website for a comprehensive overview

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Fluorescence and Chemiluminescence- HitHunter™ Enzyme Fragment Complementation (EFC) Assays
EFC technology provides a sensitive and homogeneous method for measuring analytes by enzymatically amplifying the signal. E.coli b-galactosidase (b-gal) has been split into two fragments: a large protein fragment, which acts as the enzyme acceptor (EA), and a small peptide fragment (~ 4-11 kD), which acts as the enzyme donor (ED). These fragments are inactive separately but, in solution, they rapidly recombine to form an active enzyme by the process of complementation. The recombined b-gal hydrolyses luminescent or fluorescent substrates to produce an easily detectable signal.


Choose one of the assays shown above to discover how the product can help in your studies into the lipid signaling

Need more information? Visit the HitHunter product catalogue page for a comprehensive overview

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