Understanding SPA |
Scintillation proximity assay (SPA), is an innovative approach for assay development and biochemical screening that allows the rapid and sensitive measurement of a wide variety of molecular interactions in a homogeneous system.
Need more information? Visit the SPA Website |
Understanding Cytostar-T TM plates |
Cytostar-T scintillating microplates are the same size and shape as standard 96-well microplates but have been designed specifically for cell-based assays.
These are based on the principles of scintillation proximity assay (SPA) technology and requires the use of b-emitting isotopes, such as 14C, 32P, 35S, 125I and 3H.
Need more information? Visit the Cytostar- T website for a comprehensive overview |
|
Selection guide for ADME/Tox in vitro assays and reagent
Please use the guide below to determine the best product for your needs:
Unsure about which type of assay to use? Find out more about the techniques:
Cell Proliferation Fluorescence Assay >
Distribution, metabolism and pharmaco kinetics (DMPK) >
Cell Proliferation Fluorescence Assay(SPA)
The Cell Proliferation Fluorescence Assay enables precise, fast, and simple quantitation of cell proliferation with a fluorescent endpoint. The assay utilizes nuclease digestion of DNA to allow antibody access to incorporated BrdU. Detection of bound antibody is achieved using a Cy5 labeled antibody to mouse immunoglobulin giving a fluorescent signal at sites of BrdU incorporation. Because there are no adverse affects on cell/tissue morphology, the Cell Proliferation Fluorescence Assay is ideal for use with automated sub-cellular imaging equipment and fluorescent microscopes. The assay has also been validated for use with some fluorescent plate readers.
Learn more about the Cell Proliferation Fluorescence Assay and its application in ADME/Tox studies
Return to top
Distribution, metabolism and pharmaco kinetics (DMPK)
Offer robust alternatives to conventional techniques such as equilibrium dialysis and ultrafiltration.
- SPA's proven screening technology offers sensitivity, versatility, and miniaturization capabilities.
- SPA DMPK formats available include plasma protein binding, transporter protein binding, cytochrome (CYP) P450 activity, and competition binding assays.
- Cytostar-T scintillating microplates offer real-time, non-invasive homogeneous cell-based assays suitable for tissue culture.
- Cytostar-T DMPK formats available include assays for thymidine and leucine uptake.
Learn more about the SPA/Cytostar-T Distribution, metabolism and pharmaco kinetics' (DMPK) assays and its application in ADME/Tox studies.
Return to top | 
