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Biotrak Assay Kits — Storage of antibody-coated plate

Q. If only part of the antibody coated plate from a Biotrak Assay is used, how should the plate be stored? A. The partially used antibody coated microtiter plate must be stored carefully sealed and ...

Biotrak Assays — Matrix Effects Definition

Q. What are "matrix effects" and how do they influence Biotrak immunoassay performance? A. By definition, a matrix effect is the combined effect of all components of the sample (other than the ...

Biotrak cAMP and cGMP Assays — Species cross-reactivity

Q. Will the Biotrak cAMP and cGMP assay kits work with samples from all species? A. cAMP and cGMP are ubiquitous analytes that are structurally conserved across species. Therefore, our Biotrak cAMP ...

Biotrak cAMP and cGMP EIA Kits — Kit Stability

Q. For how long is the cAMP and cGMP Biotrak EIA assay kit stable when stored as recommended? A. When stored properly, the cAMP and cGMP Biotrak EIA assay kits are stable for at least 4 weeks from ...

Biotrak cAMP and cGMP EIA kits — Sample Storage and Stability

Q. How long can samples be stored before use with the Biotrak cAMP and cGMP EIA kits? A. Due to the large complexity of most sample extracts, it is difficult to generalize cNMP (cAMP or cGMP) ...

Biotrak cAMP and cGMP EIA Kits — Storage of Reconstituted Reagents

Q. For how long can the reconstituted reagents of the Biotrak cAMP and cGMP EIA kits be stored? A. The reconstituted and undiluted reagents of the cAMP and cGMP EIA kits can be kept for up to 2 weeks ...

Biotrak cAMP and GMP Assays — Acetylation and Sensitivity

Q. Why is the acetylation protocol that is included in some Biotrak cAMP & cGMP immunoassays more sensitive than the non-acetylation protocol? A. Since the cyclic nucleotide itself is not ...

Biotrak cAMP and IP3 Assays — Cell and Tissue Culture Samples

Q. Is it possible to prepare one sample for both Biotrak cAMP and IP3 assays? A. Ice cold TCA extraction would work for both cAMP and IP3 analysis of cell culture samples. We recommend that the cell ...

Biotrak cAMP EIA — Pre-assay Room Temperature Equilibration

Q. Why do the reagents for the cAMP Biotrak EIA have to be equilibrated at room temperature before use, but the assay itself run at 4慢? A. Room temperature equilibration is important in particular ...

Biotrak cAMP EIA — Acetylation Assays using Lysis Reagents

Q. Are the acetylation reagents from the Biotrak cAMP EIA kit compatible with use of the lysis reagents? A. The use of our Direct Assay Technology's lysis reagents in combination with the acetylation ...

Biotrak cAMP EIA — Can heparin or citrate be used as an anticoagulant

Q. Can heparin or citrate be used as an anticoagulant instead of EDTA to collect blood samples for the cAMP Biotrak EIA assay kit (RPN225)? A. Use of heparin or citrate to collect blood in the cAMP ...

Biotrak cAMP EIA — Chemical cross-reactivity

Q. What is known regarding chemical crossreactivity of the Biotrak cAMP EIA kit? A. There is little interference from nucleotide analogues and PDE inhibitors based on crossreactivity of the following ...

Biotrak cAMP EIA — Interfering Compounds

Q. What chemicals and compounds interfere with the Biotrak cAMP EIA assay kit performance? A. The effect of several chemicals commonly used on performance of the Biotrak cAMP EIA assay kit is ...

Biotrak cAMP SPA Assays — Chemical crossreactivity

Q. What is known regarding the chemical crossreactivity of the cAMP SPA screening assay kit (RPA556)? A. Crossreactivity is determined by the concentration giving 50% B/B0 Chemical Cross ...

Biotrak cAMP SPA Assays— Prepareing sample without freeze drying equipment

Q. Can samples for the cAMP SPA screening assay kit (RPA556) be prepared without freeze drying equipment? A. For use with the cAMP SPA screening assay kit (RPN556) we recommend freeze drying of ...

Biotrak Cell Proliferation — Suitable Sample Types

Q: Can lymphocytes and other cells from any species be used in the Biotrak Cell Proliferation ELISA assay? A: Yes , this kit works with suspension or adherent cells from all species; also, the ...

Biotrak Cell Proliferation — Fixatives

Q: Can cells used in the Biotrak Cell Proliferation Kit be fixed with glutaraldehyde or formalin? A: Glutaraldehyde or formalin fixation may cross-link proteins too strongly, thereby inhibiting ...

Biotrak Cell Proliferation — BrdU and Other Component Stabilities

Q. What is the stability of BrdU Labeling Reagent and the other components of the Biotrak Cell Proliferation Kit and ELISA assays? A. Each batch of the Biotrak Cell Proliferation Kit and the Biotrak ...

Biotrak Cell Proliferation – How long do most cells need to be labeled with BrdU?

Q. How long do most cells need to be labelled with BrdU? A. For most applications, a 2-hour labelling is adequate. Extension of the incubation time will increase the amount of BrdU incorporated into ...

Biotrak Cell Proliferation — Does the antibody cross-react with collagen? Background OD is high

Q. When collagen coated 96-well plates were used for the Cell Proliferation ELISA assay (RPN250) the blank reading was very high (0.7 OD). Does the antibody cross-react with collagen? A. The problem ...

Biotrak Cell Proliferation — Can cells be fixed before labelling with BrdU?

Q. Can cells be fixed before labelling with BrdU? A. No, the cells have to be labelled with the BrdU before fixation as it is incorporated into the DNA as the cell divides. Click here for more ...

Biotrak Cell Proliferation — Preventing adherent cells from detaching

Q. How can I prevent adherent cells from detaching off the plate when fixing the cells as directed using the Cell Proliferation ELISA (code RPN250)? A. This may occur if surface treated plates, ...

Biotrak cAMP and cGMP EIA Kits — Assay for cAMP and cGMP from a single acetylated sample?

Q. Can an acetylated sample be assayed for both cAMP and cGMP using the RPN 225 and RPN226 Biotrak EIA assay kits? A. Unfortunately, this is not possible due to differences in the protocol for sample ...

Biotrak cAMP and cGMP EIA Kits — Assay Buffer Appearance

Q. Does a precipitate or cotton-like appearance in the assay buffer concentrate component of the cGMP or cAMP Biotrak EIA assay kit indicate it is contaminated? A. A fluffy, cottony material or ...

Biotrak cGMP EIA — Acetylation and Sensitivity

Q. Why is the acetylation protocol for the cGMP Biotrak EIA assay kit more sensitive than the non-acetylation protocol? A. Since cGMP itself is not immunogenic, our anti-cGMP antibody was raised ...

Biotrak cGMP EIA — Chemical cross-reactivity

Q. What is known regarding chemical crossreactivity of the cGMP Biotrak EIA kit? A. Crossreactivity of the following purified analogues was determined: Chemical Cross Reactant Non-acetylation ...

Biotrak cGMP EIA — Species crossreactivity

Q. What is the species crossreactivity of the Biotrak cGMP and cAMP assay kits? A. cGMP and cAMP are ubiquitous biochemical analytes that are conserved across all species; therefore, these assay kits ...

Biotrak Eicosanoid Assays — High background

Q. What factors may contribute to high backgrounds in the Biotrak Eicosanoid assays? A. If high background is seen only in samples, this suggests some matrix effect is at fault and could relate to a ...

Biotrak IP3 Assay – Sample Preparation for Cells

Q. How many cells per data point are recommended for the Biotrak IP3 assay? A. For assaying cell culture samples with the Biotrak IP3 assay kit, use between 10^5 to 10^7 cells for extraction. This is ...

Biotrak MMP-13 ELISA — Normal Serum and Plasma Values

Q. What are the expected values for MMP-13 in normal serum and plasma using the Biotrak MMP-13 ELISA? A. Tamei et al. (see belwo reference) published their findings that normal serum MMP-13 levels in ...

Biotrak MMP-2 ELISA — Differences between MMP-2 ELISA and Activity Assay Measurement

Q. What are the differences in sample readings between the MMP-2 ELISA (RPN2617) and the MMP-2 Activity Assay (RPN2631)? The pack leaflets state that a typical sample gives higher ELISA levels ...

Biotrak MMP-3 and MMP-1 Assays— Synovial Fluid Samples

Q. Have the MMP-3 or MMP-1 ELISA or Activity Assay kits been used with synovial fluid? A. The Biotrak MMP-3 ELISA has been used successfully with synovial fluid. Information relating to this can be ...

Biotrak MMP-9 Activity Assay — Reported species cross-reactivity

Q. What is the reported species cross-reactivity of the Biotrak Human MMP-9 Activity Assay (RPN2634) ? A. The human MMP-9 activity assay (RPN2634) is specific for human MMP-9, but it does cross-react ...

Biotrak MMP-9 ELISA — Serum Samples

Q. Can the Biotrak MMP-9 ELISA be used to measure MMP-9 in serum samples? A. The Biotrak MMP-9 ELISA is recommended for measurements in plasma, cell culture and tissue homogenates. In a recent ...

Biotrak MMP-9 ELISA — What parts of the MMP-9 protein do the ELISA antibodies react with?

Q. What parts of the MMP-9 protein do the ELISA antibodies react with? A. Both antibodies are raised against whole pro MMP-9. From Western Blotting experiments we know that the detection antibody ...

Biotrak MMP Activity Assays — Increasing sensitivity

Q. Is it possible to increase the sensitivity of the MMP activity assays? A. The sensitivity of MMP activity assays may be increased by simply extending the assay incubation time. The longer the ...

Biotrak MMP Activity Assays– Use of EDTA or Other Metal Chelators in Samples

Q. Will the use of EDTA and other metal chelators for sample preparation affect MMP assay readout? A. Since MMPs (matrix metalloproteinases) are zinc dependant enzymes, EDTA should not be present in ...

Biotrak MMP ELISA Assays – Recommended Storage Conditions for Plasma Samples and Stability

Q. Recommended Storage Conditions for Plasma Samples and Stability A. MMP’s are very active enzymes possessing natural autocatalytic properties and as such are prone to degradation. We recommend that ...

Biotrak MMP ELISA Assays — Species cross-reactivity

Q. What species cross-reactivity can be expected with the Biotrak MMP ELISA kits? A. ELISAs by their very nature are highly specific . Although several species have been tested, the majority of ...

Biotrak PGE2 (RPA530) — Stability of kit components

Q. What is the stability of the PGE2 (RPA530) kit components? A. All of the components in the kit with the exception of the tracer have been tested for up to 30 weeks. The methyloximation reagent has ...

Biotrak Rat Hormone Assays – Cross-reactivity with Mouse Samples

Will the Biotrak rat hormone assays work with mouse samples? Q. Do the Biotrak Rat Hormone assays have cross-reactivity for mouse samples? A. We have not validated the cross-reactivity of the Biotrak ...

Biotrak TIMP-1 ELISA — Measurable forms of TIMP-1

Q. What forms of TIMP-1 can be measured using the TIMP-1 ELISA? A. The TIMP-1 ELISA will measure free TIMP-1 and TIMP-1 that is complexed with active and pro-MMPs. It should be noted that only MMP-9 ...

Biotrak TIMP-2 ELISA — Species Crossreactivity

Q. Will the Biotrak human TIMP-2 ELISA cross-react with samples from other species? A. The TIMP-2 peptide epitopes which were used to raise antibodies are conserved across species. Therefore, the ...

Biotrak Cell Proliferation — Extending the BrdU Incubation Step

Q. Can the BrdU incubation step in the Biotrak Cell Proliferation Assay be extended for longer time? A. Yes, the incubation can be extended up to 24 hours. The incubation time needs to be determined ...

Cell Proliferation Labeling Reagent — BrdU half-life in vivo

Q. What is the in vivo half-life of BrdU in rats? A. The in vivo half-life of BrdU is 30 minutes. Click here for more information about Biotrak.

MAP Kinase SPA – Purchasing the Biotinylated MBP Substrate Separately

Q. Can I purchase the biotinylated myelin basic protein (MBP) substrate from the MAP SPA Kinase kit separately? A. The biotinylated MBP substrate is supplied in this kit (MAP Kinase SPA Assay, code ...

Biotrak MMP Activity Assays — Cloudy Wash Buffer

Q. Why does the wash buffer for the MMP Activity assays appear cloudy? A. The wash buffer for the MMP Activity assays may be cloudy due to Tween20 detergent in the concentrated solution. The ...

SPA Beads – Mechanism of Membrane binding to WGA and Polylysine SPA beads?

Q. What is the mechanism of cell membrane binding to Wheat Germ Agglutinin and Polylysine SPA beads? A. Cell membranes couple to the wheat germ agglutinin (WGA) coated SPA beads through interactions ...

TRK1000 IP3 [3H] Assay – Sample pH range tolerance

Q. What sample pH range will the Biotrak IP3 [ 3H ] assay (TRK1000) tolerate? A. The assay buffer is pH 9.0, which is the pH optimum for the IP3 assay. The assay could tolerate samples neutralized to ...

Biotrak MMP Activity Assays – Increasing the detection enzyme incubation step in the MMP Activity Assays to increase assay sensitivity

Q. Will increasing the detection enzyme incubation step in the MMP Activity Assays increase assay sensitivity? A. Absolutely, and in fact, in many cases this is necessary. More times than not, this ...

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