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Adeno-associated Virus
The conventional methods for purification of adeno associate viruses (AAV) is based on CsCl density gradient centrifugation. Such methods are difficult to scale-up and the relative low purity hampers the use in large animal models. Further pre-treatment methods such as addition of nucleases will not be compatible with future regulatory demands when future clinical use may be considered.
This scalable two step ion exchange process gives higher purity and can be used with different AAV serotypes (2 and 5). The purified virus is obtained in a buffer that is compatible with in vivo injections.
The process has the following characteristics:
- Scalable, enzyme-free process for easy transfer to GMP compliant production
- Generic for several AAV serotypes
- Gives high purity
Reusable after Cleaning in Place (CIP) with NaOH. |  |
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