GE Healthcare Life Sciences - Application notes

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Histidine-tagged protein purification
MAb
Peptides
Proteins
Rec Proteins
Application notes
GST-tagged rec protein purification
Histidine-tagged rec protein purification
Rec protein purification

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Depyrogenation of buffer or purified water

One of the most effective ways of water depyrogenation is via membrane separations, which removes contaminants and pyrogens at the molecular level using an easily-configured system. Cross flow ...

Purification of a labile, oxygen-sensitive enzyme for crystallisation and 3D structure determination

A rapid purification procedure for deacetoxycephalosporin C synthase (DAOCS), an oxygen-sensitive enzyme involved in cephalosporin/cephamycin biosynthesis, was developed using ÄKTAFPLC. The aim was ...

Rapid development of a purification process for a recombinant antigen binding fragment expressed in E. coli

This Application Note describes a systematic approach to process development using ÄKTAexplorer with UNICORN™ control. The purification of a recombinant antigen-binding fragment (Fab), expressed in ...

Separating E. coli: sometimes smaller is faster

Cross flow membrane microfiltration (MF) has become an established method for cell harvesting, cell washing and lysate clarification. Open channel, hollow fiber membranes are considered by many ...

Snags with tags: Some observations made with (His)6 -tagged proteins

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