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Selection guide - Reversed Phase Media (2)
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The effect of changing the particle diameter from 15 to 5 µm in a reversed phase chromatography separation. Separations of four peptides are shown.
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SOURCE™ 5RPC
SOURCE 15RPC
| System: | ÄKTAexplorer™ 10 XT |
| Flow Rate: | 1 ml/min |
| Sample: | Ile7 AngiotensinIII, Val4 AngiotensinIII, Angiotensin III, and Angiotensin I.
Peptides concentration 0.25 mg/ml for each peptide in 5 mM phosphoric acid. |
| Sample vol: | 10 µl |
| Solution A: | 0.1% TFA |
| Solution B: | 0.1% TFA in 50% Acetonitrile |
| Gradient: | 10-100% B over 30 min |
| Detection: | 214 nm |
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