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 Location: Protein Purification - Laboratory research > Products > Selection Guides > Columns & Media > Affinity-Isolation and Purification of Immunoglobulins
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Affinity - Coupling ligands
Affinity - Group specific
Affinity-Isolation and Purification of Immunoglobulins
Affinity-Metal Chelate (IMAC)
Affinity - Tagged proteins
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Desalting
Gel filtration
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Chromatography Columns & Media
Affinity - group specific
Affinity - antibody
Affinity - coupling
Affinity - immobilized lectin
Affinity - metal chelate
Affinity, nucleic acid, nucleotide and plasmid
Chromatofocusing
Custom Products
Desalting
Empty columns
Gel Filtration
Hydrophobic Interaction
Ion Exchange
Reversed Phase
Tagged protein purification
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Kvick cassettes and holders
Systems

Media and pre-packed columns for isolation and purification of immunoglobulines

ProductApplication areas
Pre-packed columns
HiTrap™ Protein A HP



HiTrap Protein G HP
Isolation and purification of classes, subclasses and fragments of IgG from many different species. The applications of protein G include practically all applications of protein A.

Protein G and protein A, however, have different IgG binding specificities, dependent on the origin of the IgG. Unlike protein A, protein G binds human IgG3 and has a stronger affinity to mouse IgG1 and rat IgG.
HiTrap rProtein A FFRecombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity.
HiTrap IgM Purification HPHiTrap IgM Purification HP is used for purification of monoclonal IgM from hybridoma cell culture and human IgM.
HiTrap IgY Purification HPHiTrap IgY Purification HP is used for purification of IgY from egg yolk.
Kits (including buffers)
MAbTrap™ KitMAbTrap Kit includes all necessary buffers for ten purifications using a syringe.
RPAS Purification ModuleThe RPAS Purification Module is used for purification of soluble, functional mouse single chain fragments variable (ScFv) antibodies bearing a C-terminal 13 amino acid peptide tag (E Tag).
Media
Protein A Sepharose™ CL-4B
Protein A Sepharose 4 Fast Flow
rProtein A Sepharose Fast Flow
Protein G Sepharose 4 Fast Flow
Sepharose Fast Flow (FF) media are ideal for scale up applications.

Sepharose High Performance (HP) media are ideal for high resolution purifications.