| Working principle |
- RPC separates molecules according to hydrophobicity.
|
| Used for |
- Sample concentration and desalting of peptides
- Removal of hydrophobic contaminants
- Separation of complex peptide samples
|
| Suitable stage in a purification protocol |
- Capture: (
 )
- Intermediate:
- Polishing:
  
(Number of stars indicate suitability.) |
| Separation characteristics |
|
| Very High |
|
| - General separation: Moderate
- Sample concentration/desalting: Very High
. |
|
| - General separation: High
- Sample concentration/desalting: Very High
. |
|
| - Peptides: High
- Proteins: Low |
|
| - Peptides: High
- Proteins: Low |
|
| Main optimizing parameter |
- Choice of ligand and/or running pH
|
| Special features |
- Complementary to IEX and gel filtration.
- In general not suitable for proteins
|
| Further reading |
- Applications
- Media selection guide
- Purification protocols
- Purification strategies
- (Handbooks etc.)
|
