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Ion exchange chromatography
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Basic Principles
Education Centre
About the purification of biomolecules
Purpose of purification
Developing purification protocols
How to combine purification steps
Purification development - summary
LC techniques
Affinity Chromatography
Desalting & Gel Filtration
Hydrophobic interaction chromatography
Ion exchange chromatography
Animation
Basic Principles
The Separation Mechanism
Type of ion exchangers
Elution modes
The typical Ion exchange experiment
Charge properties of proteins and peptides
Effect of running pH
Resolution in IEX
Optimisation of IEX experiments
Ion Exchange in practice
Technique Profile
What is Ion Exchange?
Reversed phase chromatography
Protein Purifier software
BioProcess™ Glossary
Basic principles in ion exchange chromatography
Contents
1.
The Separation Mechanism
- Adsorption
- Desorption
- Desorption curves
2.
Type of ion exchangers
3.
Elution modes
- Isocratic elution
- Gradient elution
- Step elution
4.
The typical IEX experiment
5.
Charge properties of proteins and peptides.
6.
Effect of Running pH
- Titration curves and resolution
- Finding the best pH
7.
Resolution in IEX
8.
Optimisation of IEX experiments
