GE Healthcare
 
GE Healthcare Life Sciences Part of GE Healthcare
labsep
Education Centre
About the purification of biomolecules
Purpose of purification
Developing purification protocols
How to combine purification steps
Purification development - summary
LC techniques
Affinity Chromatography
Desalting & Gel Filtration
Hydrophobic interaction chromatography
Animation
Basic principles
HIC in Practice
Technique Profile
What is HIC?
Ion exchange chromatography
Reversed phase chromatography
Protein Purifier software
BioProcess™ Glossary
Hydrophobic interaction chromatography profile

Working principle
  • Separates molecules according to hydrophobicity.
Used for
  • Sample concentration
  • Removal of hydrophobic contaminants.
  • Separation of complex samples.
Suitable stage in a purification protocol
  • Capture:
  • Intermediate:
  • Polishing:
(Number of stars indicate suitability.)
Separation characteristics
  • Resolution
High
  • Load capacity
- General separation: High
- Group separation: Very High
- Sample concentration: Very High
.
  • Speed
- General separation: High
- Group separation: High
- Sample concentration: High
.
  • Gentleness
Moderate to High
  • Mass yield.
Moderate to High
Main optimizing parameter
  • Choice of ligand.
Special features
  • Complementary to IEX.
  • Can be used directly after ammonium sulphate precipitation.
  • More gentle to proteins than RPC.
Further reading
    - Applications
    - Media selection guide
    - Purification protocols
    - Purification strategies
    - (Handbooks etc.)