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Desalting & Gel Filtration
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Basic Principles
Education Centre
About the purification of biomolecules
Purpose of purification
Developing purification protocols
How to combine purification steps
Purification development - summary
LC techniques
Affinity Chromatography
Desalting & Gel Filtration
Animation
Basic Principles
The separation mechanism
The gel filtration experiment
Separation modes
Characterisation of gel filtration media
Resolution in Gel filtration
Gel filtration in practice
Technique Profile
What is Gel Filtration?
Hydrophobic interaction chromatography
Ion exchange chromatography
Reversed phase chromatography
Protein Purifier software
BioProcess™ Glossary
Basic principles in Gel filtration
Contents
1.
The separation mechanism
Partitioning between the mobile and the stationary phase
Elution volumes
2.
The gel filtration experiment
Equilibration
Sample application
Elution
3.
Separation modes
The high resolution mode
The Group separation mode
4.
Characterisation of gel filtration media
Selectivity curves
- Fractionation ranges
- Exclusion limit
5.
Resolution in gel filtration
The distance between peaks (Vr2 - Vr1)
- The selectivity curve of the medium
- The length of the column
The peak width
- Uniformity of the beads and of the packed bed.
- Bead size and flow rate
