| Working principle |
- Separates molecules according to bio-specific affinity.
|
| Used for |
- One step purification of target molecules from complex samples.
- Purification of tagged recombinant proteins
- Group separations
- Removal of specific contaminants
|
| Suitable stage in a purification protocol |
- Capture:
  
- Intermediate:
  
- Polishing:
 
(Number of stars indicate suitability.) |
Separation characteristics
|
|
| Very High |
|
| High
. |
|
| Moderate
. |
|
| High |
|
| High |
|
| Main optimizing parameter |
- Choice of affinity ligand
|
| Special features |
- Very simple purification protocols
- Requires simple equipment
- Very suitable for tagged recombinant proteins
|
Further reading           |
- Applications
- Media selection guide
- Purification protocols
- Purification strategies
- (Handbooks etc.)
|
