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Protein Array Formats
A number of different formats for protein arrays are available depending on the application required. In general, protein arrays or “chips” require highly specific binders bound to a solid planar support and coupled to a method of detection.
The solid surface, such as a microscope slide, has a limited number of protein binding elements, each with specific affinity for an individual protein, immobilized in a medium density format for profiling known/defined marker proteins.
By using binding elements of well-defined and limited specificity immobilized in a defined location on an array, positional information (and hence protein identity) can be obtained.
Multicolor fluorescent labelling of samples is carried out with CyDye fluors.
Accurate quantitative differential analysis can also be performed, since differences in protein levels within complex samples can be easily measured. | |
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